Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceTESSSRGPYHPSECCFTYTTYKIPRQRIMDYYETNSQCSKPGIVFITKRG HSVCTNPSDKWVQDYIKDMKEN
    • Molecular weight8 kDa
    • Additional sequence informationHCC-1 Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 8411 Dalton.

Specifications

Our Abpromise guarantee covers the use of ab73836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity: The Biological activity is calculated by its ability to chemoattract Human monocytes at 5-20 ng/ml.
  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab73836 is purified by proprietary chromatographic techniques. Purity is greater than 97.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20mM PBS, 150mM Sodium chloride, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Upon reconstitution ab73836 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

General Info

  • Alternative names
    • CC-1
    • CC-3
    • CCL14
    • CCL14_HUMAN
    • chemokine (C-C motif) ligand 14
    • Chemokine CC-1/CC-3
    • chemokine CC1
    • chemokine CC3
    • chemokine HCC1
    • chemokine HCC3
    • CKb1
    • HCC-1(1-74)
    • HCC-1(9-74)
    • HCC-1/HCC-3
    • HCC-3
    • HCC1
    • HEMOFILTRATE CC CHEMOKINE 1
    • MCIF
    • NCC-2
    • NCC2
    • new CC chemokine 2
    • SCYA14
    • SCYL2
    • small inducible cytokine subfamily A (Cys-Cys), member 14
    • Small-inducible cytokine A14
    • SY14
    see all
  • FunctionHas weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
  • Tissue specificityExpressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.
    HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human CCL14 protein (ab73836)

ab73836 has not yet been referenced specifically in any publications.

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