Recombinant human CCNO protein (Active) (ab177582)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence 1
    • Accession
    • Species
      Human
    • Sequence
      MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIR EISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIP LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLAR AFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRR ALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSK VVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
    • Molecular weight
      58 kDa including tags
    • Amino acids
      1 to 298
    • Tags
      proprietary tag N-Terminus
    • Additional sequence information
      NM_001798
    Amino Acid Sequence 2
    • Accession
    • Species
      Human
    • Sequence
      MVTPCPTSPSSPAARAGRRDNDQNLRAPVKKSRRPRLRRKQPLHPLNPCP LPGDSGICDL FESPSSGSDGAESPSAARGGSPLPGPAQPVAQLDLQTF RDYGQSCYAFRKAQESHFHPRE ALARQPQVTAESRCKLLSWLIPVHRQ FGLSFESLCLTVNTLDRFLTTTPVAADCFQLLGV TSLLIACKQVEVHP PRVKQLLALCCGAFSRQQLCNLECIVLHKLHFTLGAPTISFFLEHF TH ARVEAGQAEASEALEAQALARGVAELSLADYAFTSYSPSLLAICCLALAD RMLRVSRP VDLRLGDHPEAALEDCMGKLQLLVAINSTSLTHMLPVQIC EKCSLPPSSK
    • Molecular weight
      68 kDa including tags
    • Amino acids
      1 to 350
    • Tags
      proprietary tag N-Terminus
    • Additional sequence information
      NM_021147

Specifications

Our Abpromise guarantee covers the use of ab177582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of Cdk2/ Cyclin O was determined to be 46 nmol/min/mg.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity
    >90% by SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CCNO
    • CCNO_HUMAN
    • CCNU
    • Cyclin domain containing
    • Cyclin like uracil DNA glycosylase
    • Cyclin O
    • Cyclin U
    • Cyclin-O
    • DGU
    • EC 3.2.2.
    • UDG2
    • UNG2
    • Uracil DNA glycolylase 2
    • Uracil DNA glycosylase cyclin like
    see all
  • Sequence similarities
    Belongs to the cyclin family.
  • Developmental stage
    Maximum levels during G(1) phase. Levels decrease through S and G(2) phases.
  • Information by UniProt

Images

  • The specific activity of Cdk2/ Cyclin O was determined to be 46 nmol/min/mg.

  • SDS-PAGE analysis of ab177852 which was determined to be >90% pure by densitometry. Cdk2 is approximately 58 kDa and Cyclin O is approximately 68 kDa.

References

ab177582 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab177582.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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