Recombinant Human CD18 protein (ab114283)

Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MLGLRPPLLALVGLLSLGCVLSQECTKFKVSSCRECIESGPGCTWCQKLN FTGPGDPDSIRCDTRPQLLMRGCAADDIMDPTSLAETQEDHNGGQKQLSP QKVTLYLRPGQAAAFNVTFRRAKGYPIDLYYLMDLSYSMLDDLRNVKKLG GDLLRALNEITESGRIGFGSFVDKTVLPFVNTHPDKLRNPCPNKEKECQP PFAFRHVLKLTNNSNQFQTEVGKQLISGNLDAPEGGLDAMMQVAACPEEI GWRNVTRLLVFATDDGFHFAGDGKLGAILTPNDGRCHLEDNLYKRSNEFD YPSVGQLAHKLAENNIQPIFAVTSRMVKTYEKLTEIIPKSAVGELSEDSS NVVHLIKNAYNKLSSRVFLDHNALPDTLKVTYDSFCSNGVTHRNQPRGDC DGVQINVPITFQVKVTATECIQEQSFVIRALGFTDIVTVQVLPQCECRCR DQSRDRSLCHGKGFLECGICRCDTGYIGKNCECQTQGRSSQELEGSCRKD NNSIICSGLGDCVCGQCLCHTSDVPGKLIYGQYCECDTINCERYNGQVCG GPGRGLCFCGKCRCHPGFEGSACQCERTTEGCLNPRRVECSGRGRCRCNV CECHSGYQLPLCQECPGCPSPCGKYISCAECLKFEKGPFGKNCSAACPGL QLSNNPVKGRTCKERDSEGCWVAYTLEQQDGMDRYLIYVDESRECVAGPN IAAIVGGTVAGIVLIGILLLVIWKALIHLSDLREYRRFEKEKLKSQWNND NPLFKSATTTVMNPKFAES
    • Molecular weight
      111 kDa including tags
    • Amino acids
      1 to 769

Specifications

Our Abpromise guarantee covers the use of ab114283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
    This protein is best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.3% Glutathione

General Info

  • Alternative names
    • 95 subunit beta
    • Beta 2 (antigen CD18 (p95)
    • CD 18
    • CD18
    • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor)
    • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta
    • Cell surface adhesion glycoproteins LFA-1/CR3/p150
    • Complement component 3 receptor 3 and 4 subunit
    • Complement receptor C3 beta subunit
    • Complement receptor C3 subunit beta
    • Integrin beta 2
    • Integrin beta chain beta 2
    • Integrin beta-2
    • Integrin beta-2 (ITGB2)
    • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
    • ITB2_HUMAN
    • ITGB2
    • LAD
    • LCAMB
    • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C
    • Leukocyte cell adhesion molecule CD18
    • LFA 1
    • LFA1
    • Lymphocyte function associated antigen 1
    • Lymphocyte function associated antigen 1 (LFA1)
    • MAC 1
    • MAC1
    • Macrophage antigen 1 (mac1) beta
    • MF17
    • MFI7
    • OTTHUMP00000115278
    • OTTHUMP00000115279
    • OTTHUMP00000115280
    • OTTHUMP00000115281
    • OTTHUMP00000115282
    see all
  • Function
    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
  • Involvement in disease
    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  • Cellular localization
    Membrane.
  • Information by UniProt

Recombinant Human CD18 protein images

  • 12.5% SDS-PAGE analysis of CD18 protein (ab114283). Stained with Coomassie Blue.

References for Recombinant Human CD18 protein (ab114283)

ab114283 has not yet been referenced specifically in any publications.

Product Wall

Application
Other
The effect of recombinant protein on cell viability is assessed using MTS viability determination method on cultured CHO-M2 cells incubated 42h in presence of different concentrations (45.5 – 22.7 – 11.4 – 5.7 – 2.8 – 1.4 nM) of rCD18.

CONCLUSION
The viability is similar for the different concentration of rCD18. It doesn’t affect cell viability.
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Submitted Aug 26 2016

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