Recombinant human CD300 antigen protein (Active) (ab185267)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      44 kDa including tags
    • Amino acids
      18 to 178
    • Additional sequence information
      Extracellular domain (AAH32352), fused with Fc fragment of human IgG1 at the C-terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab185267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.  The ED50 for this effect is typically 2-6 µg/ml.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.50
    Constituents: 0.6% Tris, 0.75% Glycine, 3% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute the lyophilized product in 100 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

General Info

  • Alternative names
    • CD300 antigen-like family member A
    • CD300a
    • CD300a antigen
    • CD300a molecule
    • CLM-8
    • CLM8_HUMAN
    • CMRF 35 H9
    • CMRF-35-H9
    • CMRF35-H
    • CMRF35-H9
    • CMRF35-like molecule 8
    • CMRF35H
    • CMRF35H leukocyte immunoglobulin like receptor
    • CMRF35H9
    • IgSF12
    • Immunoglobulin superfamily member 12
    • Inhibitory receptor protein 60
    • IRC1
    • IRC1/IRC2
    • IRC2
    • IRp60
    • Leukocyte membrane antigen
    • Mast cell derived paired immunoglobulin like receptor 1
    • NK inhibitory receptor
    • Polymeric immunoglobulin receptor 4
    see all
  • Function
    Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation.
  • Tissue specificity
    Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.
  • Sequence similarities
    Belongs to the CD300 family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

Images

  • DTT-reduced (+) and non-reduced (-) SDS-PAGE analysis of ab185267, and staining overnight with Coomassie Blue. As a result of glycosylation, DTT-reduced Protein migrates as 52-66 kDa and non-reduced protein migrates as 105-130 kDa.

References

ab185267 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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