The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 of ab83917 is typically 1.0-1.5 ug/ml as measured by its ability to neutralize CD40 ligand mediated proliferation of T47-D cells.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
B cell associated molecule CD40
B cell surface antigen CD40
B cell-associated molecule
B-cell surface antigen CD40
CD40 antigen (TNF receptor superfamily member 5)
CD40 molecule, TNF receptor superfamily member 5
CD40 type II isoform
Nerve growth factor receptor related B lymphocyte activation molecule
TNF receptor superfamily member 5
Tumor necrosis factor receptor superfamily , member 5
Tumor necrosis factor receptor superfamily member 5
Tumor necrosis factor receptor superfamily member 5 precursor
Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a
Receptor for TNFSF5/CD40LG.
B-cells and in primary carcinomas.
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Recombinant human CD40 protein (Fc Chimera Active) images
Functional Studies - CD40 protein (Fc Chimera Active) (ab83917)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - CD40 protein (Fc Chimera Active) (ab83917)
1D SDS-PAGE of ab83917 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83917
Lane 2: ab83917 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83917 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
Western blot - CD40 protein (Fc Chimera Active) (ab83917)
Anti-CD40 antibody (ab13545) at 1/1000 dilution + Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique