Recombinant human CD40L protein (Soluble) (ab51956)



  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP29965
    • SpeciesHuman
    • Molecular weight16 kDa
    • Amino acids113 to 261
    • Additional sequence informationFull length soluble form.


Our Abpromise guarantee covers the use of ab51956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity: Determined by the stimulation of IL-12 induction by human peripheral blood mononuclear cells (PBMC) and the stimulation of IL-8 induction by human PBMC. The expected ED50 for this effect is 5-10 ng/ml.Note: Results may vary with PBMC donors.
  • Applications


    Functional Studies

  • Purity> 95 % SDS-PAGE.
    >98% by SDS-PAGE and HPLC analyses
  • FormLyophilised
  • Additional notesThe reconstituted solution can be diluted into aqueous buffers or stored at 4°C for 1 week or –20°C for future use.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Lyophilized from 10mM Sodium phosphate, pH 7.5
    Endotoxin level is <0.1 ng per µg of CD40L

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in 5-10 mM Sodium Phosphate (pH 7.5) to a concentration of 0.1-1.0 mg/ml.

General Info

  • Alternative names
    • CD 40L
    • CD154
    • CD40 antigen ligand
    • CD40 ligand
    • CD40 ligand, soluble form
    • CD40-L
    • CD40L
    • CD40L_HUMAN
    • CD40LG
    • gp39
    • hCD40L
    • HIGM1
    • IGM
    • IMD3
    • T B cell activating molecule
    • T BAM
    • T-cell antigen Gp39
    • TNF-related activation protein
    • TNFSF5
    • TrAP
    • Tumor necrosis factor (ligand) superfamily member 5
    • Tumor necrosis factor ligand superfamily member 5
    see all
  • FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
  • Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt

References for Recombinant human CD40L protein (Soluble) (ab51956)

ab51956 has not yet been referenced specifically in any publications.

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