Recombinant human CD44 protein (Active) (ab221334)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, HPLC, SDS-PAGE
Description
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Product name
Recombinant human CD44 protein (Active)
See all CD44 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized ab221334 at 5 μg/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 10-156 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind ab221334 with a linear range of 0.156-2.5 μg/mL.
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Purity
> 95 % SDS-PAGE.
>90% as determined by SEC-HPLC. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QIDLNITCRF AGVFHVEKNG RYSISRTEAA DLCKAFNSTL PTMAQMEKAL SIGFETCRYG FIEGHVVIPR IHPNSICAAN NTGVYILTSN TSQYDTYCFN ASAPPEEDCT SVTDLPNAFD GPITITIVNR DGTRYVQKGE YRTNPEDIYP SNPTDDDVSS GSSSERSSTS GGYIFYTFST VHPIPDEDSP WITDSTDRIPP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK -
Predicted molecular weight
49 kDa including tags -
Amino acids
21 to 220 -
Tags
Fc tag C-Terminus -
Additional sequence information
ab221334 has a Human IgG1 Fc tag (Uniprot# P01857, aa 110-330) at the C-terminus.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab221334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
HPLC
SDS-PAGE
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Form
Lyophilized -
Additional notes
No activity loss is observed after storage at:
- -20 to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, Glycine, Trehalose, L-Arginine, Sodium chloride
Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- LHR
- BA-1
- CD 44
see all -
Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
Images
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Immobilized ab221334 at 5 μg/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 10-156 ng/mL.
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SDS-PAGE of reduced ab221334 stained overnight with Coomassie Blue. Due to glycosylation, the protein migrates at 76 kDa on a under reducing conditions.
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Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind ab221334 with a linear range of 0.156-2.5 μg/mL.
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SEC-HPLC analysis of ab221334. The purity of ab221334 was determined to be greater than 90%.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab221334 has not yet been referenced specifically in any publications.