Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      584 to 1256

Specifications

Our Abpromise guarantee covers the use of ab42584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 26 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mM DTT, 100 mM NaCl, 50 mM pNPP. The specific activity of CD45 was determined using pNPP. Enzyme reaction condition: 20 mM pNPP, 2 min incubation at 30°C, 2µg/ml enzyme.
  • Applications

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Protein was expressed in a Baculovirus Sf9 expression system. Expected molecular weight 75kDa.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 75mM Sodium chloride, 5mM DTT, 25mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • B220
    • CD 45
    • CD45
    • CD45 antigen
    • CD45R
    • GP180
    • L-CA
    • LCA
    • Leukocyte common antigen
    • loc
    • Ly-5
    • LY5
    • Ly5, homolog of
    • Lyt-4
    • OTTHUMP00000033813
    • OTTHUMP00000033816
    • OTTHUMP00000033817
    • OTTHUMP00000038574
    • Protein tyrosine phosphatase receptor type c polypeptide
    • Protein tyrosine phosphatase, receptor type C
    • protein tyrosine phosphatase, receptor type, C
    • Protein tyrosine phosphatase, receptor type, c polypeptide
    • Ptprc
    • PTPRC_HUMAN
    • Receptor-type tyrosine-protein phosphatase C
    • T200
    • T200 glycoprotein
    • T200 leukocyte common antigen
    see all
  • Function
    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in disease
    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • Domain
    The first PTPase domain interacts with SKAP1.
  • Post-translational
    modifications
    Heavily N- and O-glycosylated.
  • Cellular localization
    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

References

ab42584 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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