The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% by SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
CD62 antigen-like family member P
Granule membrane protein 140
Granulocyte membrane protein
Leukocyte endothelial cell adhesion molecule 3
Leukocyte-endothelial cell adhesion molecule 3
Platelet activation dependent granule-external membrane protein
Platelet alpha granule membrane protein
Selectin P (granule membrane protein 140kDa, antigen CD62)
FunctionCa(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
Tissue specificityStored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Involvement in diseaseDefects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Sequence similaritiesBelongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 9 Sushi (CCP/SCR) domains.
Lane 1 ab83582; Lane 2 ab83582 treated with PNGase F to remove potential Nlinked glycans; Lane 3 ab83582 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
References for Recombinant Human CD62P protein (ab83582)
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