Recombinant human Cdc25C protein (ab105911)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      84 kDa including tags
    • Amino acids
      1 to 473

Specifications

Our Abpromise guarantee covers the use of ab105911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The Specific activity of ab105911 was determined to be 22 nmol/min/mg.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 70 % SDS-PAGE.
    Purity was determined to be >70% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CDC 25
    • Cdc 25C
    • CDC25
    • CDC25C
    • Cell division cycle 25 homolog C
    • Cell division cycle 25C
    • Cell division cycle 25C protein
    • Dual specificity phosphatase Cdc25C
    • M phase inducer phosphatase 3
    • M-phase inducer phosphatase 3
    • Mitosis inducer CDC25
    • MPIP3
    • MPIP3_HUMAN
    • Phosphotyrosine phosphatase
    • PPP1R60
    • protein phosphatase 1, regulatory subunit 60
    see all
  • Function
    Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • Sequence similarities
    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Developmental stage
    Expressed predominantly in G2 phase.
  • Post-translational
    modifications
    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • Phosphatase Assay demonstrating specific activity of ab105911.
  • SDS-PAGE showing ab105911 at approximately 84kDa.

References

ab105911 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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