Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEP YTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPE ITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLK AVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRC
    • Molecular weight48 kDa
    • Amino acids1 to 188
    • TagsHis tag N-Terminus , GST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab43599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • CDC42
    • CDC42_HUMAN
    • CDC42Hs
    • Cell division control protein 42 homolog
    • Cell division cycle 42
    • Cell division cycle 42 (GTP binding protein 25kDa)
    • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))
    • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))
    • G25K
    • G25K GTP-binding protein
    • Growth regulating protein
    • GTP binding protein 25kDa
    • Small GTP binding protein CDC42
    • TKS
    see all
  • FunctionPlasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
  • Post-translational
    modifications
    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localizationCell membrane.
  • Information by UniProt

Recombinant Human CDC42 protein images

  • Recombinant Human CDC42 protein (ab43599)

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    The GST+His tag at the N-terminus gives almost ~27kDa increase in molecular weight of the CDC42 protein.

References for Recombinant Human CDC42 protein (ab43599)

ab43599 has not yet been referenced specifically in any publications.

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