Recombinant Human CDK2 + CCNE1 protein (ab85836)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab85836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

General Info

  • Alternative names
    • CCNE
    • CCNE 1
    • CCNE1
    • Cdc2 related protein kinase
    • Cdk 2
    • CDK2
    • Cell division kinase 2
    • Cell division protein kinase 2
    • Cyclin dependent kinase 2
    • Cyclin E1
    • Cyclin Es
    • Cyclin Et
    • CyclinE
    • G1/S specific cyclin E
    • G1/S specific cyclin E1
    • p33 protein kinase
    • p33(CDK2)
    see all
  • Relevance
    CDK2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. CCNE1 (Cyclin E) is a regulatory subunit of CDK2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
  • Cellular localization
    CCNE1: Nuclear.

Images

  • SDS-PAGE showing ab85836 at approximately 58 kDa (CDK2) and 73 kDa (CCNE1).

References

ab85836 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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