Recombinant human CDK4 + CCND3 protein (ab85646)


  • Product name
    Recombinant human CDK4 + CCND3 protein
  • Protein length
    Full length protein


  • Nature
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species


Our Abpromise guarantee covers the use of ab85646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The Specific activity of ab85646 was determined to be 16 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies


  • Form
  • Additional notes

    ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CCND 3
    • CCND3
    • Cdk 4
    • CDK4 protein
    • Cell division kinase 4
    • Cell division protein kinase 4
    • CMM 3
    • CMM3
    • Crk3
    • Cyclin D3
    • Cyclin dependent kinase 4
    • CyclinD3
    • D3 type cyclin
    • G1 S specific cyclin D3
    • G1/S specific cyclin D3
    • Melanoma cutaneous malignant 3
    • MGC14458
    • p34 cdk4
    • PSK J3
    see all
  • Relevance
    CDK4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. CDK4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). CDK4 was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. CCND3 (Cyclin D3) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. CCND3 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. It has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb.
  • Cellular localization
    CCND3: Cytoplasm. Nucleus.

Recombinant human CDK4 + CCND3 protein images

  • The Specific activity of ab85646 was determined to be 16 nmol/min/mg.
  • SDS-PAGE showing ab85646 at approximately 58kDa (both proteins).

References for Recombinant human CDK4 + CCND3 protein (ab85646)

ab85646 has not yet been referenced specifically in any publications.

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