Recombinant Human CDK5R1(p25) protein. (ab125653)

Overview

  • Product name
    Recombinant Human CDK5R1(p25) protein.
  • Protein length
    Full length protein
  • Description
    Recombinant Human CDK5R1 protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      49 kDa including tags
    • Amino acids
      98 to 307

Specifications

Our Abpromise guarantee covers the use of ab125653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names
    • CD5R1_HUMAN
    • CDK5 activator 1
    • CDK5R1
    • Cyclin-dependent kinase 5 activator 1
    • Cyclin-dependent kinase 5 regulatory subunit 1
    • p23
    • p25
    • p35
    • Tau protein kinase II 23 kDa subunit
    • TPKII regulatory subunit
    see all
  • Function
    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • Tissue specificity
    Brain and neuron specific.
  • Sequence similarities
    Belongs to the cyclin-dependent kinase 5 activator family.
  • Post-translational
    modifications
    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • Cellular localization
    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab125653.

References

ab125653 has not yet been referenced specifically in any publications.

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