Recombinant human CDK6 + CCND1 protein (ab84559)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CDK6 + CCND1 protein -
Biological activity
Specific activity of CDK6 + CCND1 was determined to be 9.3 nmol/min/mg as per activity assay protocol.
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Purity
> 85 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab84559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- B cell CLL/lymphoma 1
- BCL 1 oncogene
- BCL1
see all -
Relevance
CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors (1). The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein (2). CCND1 is essential for the control of the cell cycle at the G1/S (start) transition
Images
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The specific activity of CDK6 + CCND1 (ab84559) was determined to be 1.7 nmol/min/mg as per activity assay protocol and was equivalent to 8 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab84559
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SDS PAGE analysis of ab84559
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Purity of CDK6 + CCND1 was determined to be >85% by densitometry. CDK6 approx. MW 40kDa and CCND1 approx. MW 61kDa.
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Specific activity of CDK6 + CCND1 was determined to be 9.3 nmol/min/mg as per activity assay protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84559 has not yet been referenced specifically in any publications.