Recombinant human CDK6 + CCND1 protein (ab84559)

Overview

  • Product nameRecombinant human CDK6 + CCND1 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab84559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity of CDK6 + CCND1 was determined to be 9.3 nmol/min/mg as per activity assay protocol.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Additional notes

    ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • B cell CLL/lymphoma 1
    • BCL 1 oncogene
    • BCL1
    • cell division protein kinase 6
    • cyclin D1 1
    • cyclin dependent kinase 6
    • G1 S specific cyclin D1
    • PRAD1
    • PRAD1 oncogene
    • Serine threonine protein kinase PLSTIRE
    see all
  • RelevanceCDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors (1). The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein (2). CCND1 is essential for the control of the cell cycle at the G1/S (start) transition

Recombinant human CDK6 + CCND1 protein images

  • Purity of CDK6 + CCND1 was determined to be >85% by densitometry. CDK6 approx. MW 40kDa and CCND1 approx. MW 61kDa.
  • Specific activity of CDK6 + CCND1 was determined to be 9.3 nmol/min/mg as per activity assay protocol.

References for Recombinant human CDK6 + CCND1 protein (ab84559)

ab84559 has not yet been referenced specifically in any publications.

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