Recombinant Human CDK6 + CCND3 protein (ab89010)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human CDK6 + CCND3 protein
See all CDK6 + CCND3 proteins and peptides -
Purity
> 90 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab89010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
General Info
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Alternative names
- CCND 3
- CCND3
- CDK 6
see all -
Relevance
CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 are members of a growing superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating the activity of cyclin-dependent kinases (cdks). All three known members of the D-type subfamily form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6. -
Cellular localization
CCND3: Cytoplasm. Nucleus
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab89010 has not yet been referenced specifically in any publications.