Overview

  • Product name
    Recombinant human CDK9+CyclinK protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Tags
      GST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab70320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab70320 was determined to be 18 nmol /min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Additional notes

    ab204873 (AKT1 + PKN2 peptide substrate) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CCNK
    • CDC2L4
    • CDK 9
    • CPR4
    • CTK1
    • cyclin dependent kinase 9
    • Cyclin K
    • PITALRE
    • TAK
    see all
  • Relevance
    Cdk9 is a member of the cyclin-dependent kinase pair (CDK9/Cyclin K) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). The cdk9/cyclin k complex also has a kinase activity towards the CTD of RNAP II and can substitute for P-TEFB in vitro. In vitro, it phosphorylates retinoblastoma and myelin basic protein. Cyclin K (CCNK) belongs to the highly conserved cyclin family and is thought to play a role in transcriptional regulation. CCNK and its kinase partner CDK9 are subunits of the transcription elongation factor pTEFB. CCNK may play a dual role in regulating CDK and RNA polymerase II activities.
  • Cellular localization
    CDK9: Nuclear.

Images

  • SDS-PAGE showing CDK9/CyclinK at approximaltely 67kDa.
  • Kinase Assay demonstrating CDK9/CyclinK specific activity.

References

This product has been referenced in:
  • Mikula M  et al. Halogenated imidazole derivatives block RNA polymerase II elongation along mitogen inducible genes. BMC Mol Biol 11:4 (2010). Read more (PubMed: 20078881) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70320.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up