Overview

  • Product nameRecombinant Human CDT2 protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids1 to 231

Specifications

Our Abpromise guarantee covers the use of ab80154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

  • ReconstitutionRedissolve the powder with 1ml sterile water will restore to the original concentration 1mg/ml.

General Info

  • Alternative names
    • Lethal(2) denticleless protein homolog
    • CDW1
    • DCAF2
    • DDB1 and CUL4 associated factor 2
    • Ddb1- and Cul4-associated factor 2
    • Denticleless homolog
    • Denticleless homolog (Drosophila)
    • Denticleless protein homolog
    • Dtl
    • DTL_HUMAN
    • L2DTL
    • Lethal(2) denticleless protein homolog
    • RA regulated nuclear matrix associated protein
    • RAMP
    • Retinoic acid regulated nuclear matrix associated protein
    • Retinoic acid-regulated nuclear matrix-associated protein
    see all
  • FunctionSubstrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
  • Tissue specificityExpressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the WD repeat cdt2 family.
    Contains 7 WD repeats.
  • Developmental stageExpressed in all fetal tissues examined, included brain, lung, liver, and kidney.
  • Post-translational
    modifications
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C).
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Nucleus membrane. Cytoplasm > cytoskeleton > centrosome. Nuclear matrix-associated protein. Translocates from the interphase nucleus to the metaphase cytoplasm during mitosis.
  • Information by UniProt

Recombinant Human CDT2 protein images

  • 20% SDS PAGE showing ab80154 at approximately 31 kDa. Coomassie blue staining.

References for Recombinant Human CDT2 protein (ab80154)

ab80154 has not yet been referenced specifically in any publications.

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