Overview

  • Product name
    Recombinant Human CENPB protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab73636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    ab73636 reacts with Scleroderma patient sera.
  • Applications

    Western blot

    ELISA

  • Purity
    > 90 % SDS-PAGE.
    ab73636 is purified by proprietary chromatographic techniques.
  • Form
    Liquid
  • Additional notes
    ab73636 reacts with Scleroderma patient sera.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 250mM Sodium chloride, 20mM HEPES, pH 7.6

General Info

  • Alternative names
    • CENP B
    • CENP-B
    • CENPB
    • CENPB_HUMAN
    • centromere autoantigen B
    • Centromere protein B
    • Centromere protein B, 80kDa
    • Major centromere autoantigen B
    see all
  • Function
    Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
  • Sequence similarities
    Contains 1 HTH CENPB-type DNA-binding domain.
    Contains 1 HTH psq-type DNA-binding domain.
  • Cellular localization
    Nucleus. Chromosome > centromere.
  • Information by UniProt

References

This product has been referenced in:
  • Mohibi S  et al. Alteration/Deficiency in Activation 3 (ADA3) Protein, a Cell Cycle Regulator, Associates with the Centromere through CENP-B and Regulates Chromosome Segregation. J Biol Chem 290:28299-310 (2015). Read more (PubMed: 26429915) »
  • Favoino E  et al. Autoantibodies recognizing the amino terminal 1-17 segment of CENP-A display unique specificities in systemic sclerosis. PLoS One 8:e61453 (2013). WB . Read more (PubMed: 23613856) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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