The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityDetermined by its ability to chemoattract human immature dendritic cells using a concentration range of 1.0-100.0 ng/ml.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. Purity > 98% by SDS-PAGE gel and HPLC analyses.
Sterile filtered through a 0.2 micron filter.
Determined by its ability to chemoattract human immature dendritic cells using a concentration range of 1.0-100.0 ng/ml.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
ReconstitutionReconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Tissue specificityHighly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon.