Recombinant human Chemerin protein (ab124314)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, Functional Studies
Description
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Product name
Recombinant human Chemerin protein
See all Chemerin proteins and peptides -
Biological activity
ab124314 has full biological activity when compared to standards. The ED50, as determined by its ability to chemoattract Human Chem23R transfected BaF3 mouse pro-B cells, is typically 4-20 ng/mL.
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Purity
>= 98 % SDS-PAGE.
Purity by SDS-PAGE: =98%
Purity by HPLC: =98% -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MELTEAQRRG LQVALEEFHK HPPVQWAFQE TSVESAAVDTP FPAGIFVRLE FKLQQTSRK RDWKKPECKV RPNGRKRKCL ACIKLGSEDK VLGRLVHCPI ETQVLREAEE HQETQCLRVQ RAGEDPHSFY FPGQFAFS -
Predicted molecular weight
16 kDa -
Amino acids
21 to 157
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Associated products
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Matched antibody pairs
Specifications
Our Abpromise guarantee covers the use of ab124314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
HPLC
Functional Studies
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Form
Lyophilized -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 4002 Chemerin, human recombinant. 4002-25 is the same size as the 25 µg size of ab124314.
Endotoxin level: <0.01 ng/µg.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituent: 0.2% Trifluoroacetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. When reconstituting the product, gently pipette and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1mg/ml, which can be further diluted into other aqueous solutions.
General Info
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Alternative names
- HP10433
- RAR responsive protein TIG2
- RAR-responsive protein TIG2
see all -
Tissue specificity
Highly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124314 has been referenced in 1 publication.
- Zhang Y et al. Chemerin suppresses neuroinflammation and improves neurological recovery via CaMKK2/AMPK/Nrf2 pathway after germinal matrix hemorrhage in neonatal rats. Brain Behav Immun 70:179-193 (2018). PubMed: 29499303