Recombinant human Chk2 protein (ab90275)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab90275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity> 100,000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide KKKVSRSGLYRSPSMPENLNRPR per min at 30 °C).
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purified by Ni-NTA agarose chromatography.
  • FormLiquid
  • Additional notes> 100,000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide KKKVSRSGLYRSPSMPENLNRPR per min at 30 °C).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: Protease inhibitor cocktail, 50mM Tris HCl, 270mM Sucrose, 150mM Sodium chloride, 10mM DTT, 0.1mM EGTA, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CDS 1
    • Cds1
    • Cds1 homolog
    • Checkpoint kinase 2
    • Checkpoint like protein CHK2
    • CHEK 2
    • Chek2
    • Chk 2
    • CHK2 checkpoint homolog
    • CHK2 checkpoint homolog (S. pombe)
    • CHK2_HUMAN
    • hCds1
    • HuCds 1
    • LFS 2
    • LFS2
    • PP1425
    • RAD 53
    • RAD53
    • Rad53 homolog
    • Serine/threonine protein kinase Chk2
    • Serine/threonine-protein kinase Chk2
    see all
  • FunctionRegulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
  • Tissue specificityHigh expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
  • Involvement in diseaseDefects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by PLK4.
  • Cellular localizationNucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt

References for Recombinant human Chk2 protein (ab90275)

ab90275 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"