Recombinant human CK1 epsilon protein (ab104024) (ab104024)

Overview

  • Product name
    Recombinant human CK1 epsilon protein (ab104024)
  • Protein length
    Full length protein
  • Description
    Recombinant human CK1 epsilon protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      72 kDa including tags
    • Amino acids
      1 to 416

Specifications

Our Abpromise guarantee covers the use of ab104024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The Specific activity of ab104024 was determined to be 46 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    > 75 % SDS-PAGE.
    Purity was determined to be >75% by densitometry.
  • Form
    Liquid
  • Additional notes

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Casein kinase 1 epsilon
    • Casein kinase I
    • Casein kinase I epsilon
    • Casein kinase I isoform epsilon
    • CKI epsilon
    • CKI-epsilon
    • CKIe
    • CKIepsilon
    • CSNK 1E
    • CSNK1E
    • DBT
    • Doubletime, Drosophila, homolog of
    • EC 2.7.11.1
    • epsilon isoform
    • HCK IE
    • KC1E
    • KC1E_HUMAN
    • kinase CK1 epsilon
    • MGC 10398
    see all
  • Function
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • Tissue specificity
    Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • The Specific activity of ab104024 was determined to be 46 nmol/min/mg.
  • SDS-PAGE showing ab104024 at approximately 72kDa.

References

ab104024 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please see the requested protocol below. Please contact me again if you have any further questions.

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Kinase assay protocol for CK1-epsilon, Abcam ab104024

Thaw [32P]-ATP assay cocktail in a designated radioactive worki...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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