Recombinant human CK1 epsilon protein (ab104024)

Overview

  • Product nameRecombinant human CK1 epsilon protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionP49674
    • SpeciesHuman
    • Molecular weight72 kDa including tags
    • Amino acids1 to 416

Specifications

Our Abpromise guarantee covers the use of ab104024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe Specific activity of ab104024 was determined to be 46 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 75 % SDS-PAGE.
    Purity was determined to be >75% by densitometry.
  • FormLiquid
  • Additional notes

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Casein kinase 1 epsilon
    • Casein kinase I
    • Casein kinase I epsilon
    • Casein kinase I isoform epsilon
    • CKI epsilon
    • CKI-epsilon
    • CKIe
    • CKIepsilon
    • CSNK 1E
    • CSNK1E
    • DBT
    • Doubletime, Drosophila, homolog of
    • EC 2.7.11.1
    • epsilon isoform
    • HCK IE
    • KC1E
    • KC1E_HUMAN
    • kinase CK1 epsilon
    • MGC 10398
    see all
  • FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • Tissue specificityExpressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt

Recombinant human CK1 epsilon protein images

  • The Specific activity of ab104024 was determined to be 46 nmol/min/mg.
  • SDS-PAGE showing ab104024 at approximately 72kDa.

References for Recombinant human CK1 epsilon protein (ab104024)

ab104024 has not yet been referenced specifically in any publications.

Product Wall

Please see the requested protocol below. Please contact me again if you have any further questions.

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Kinase assay protocol for CK1-epsilon, Abcam ab104024

Thaw [32P]-ATP assay cocktail in a designated radioactive worki...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"