Recombinant Human Claspin protein (ab125977)

Overview

  • Product nameRecombinant Human Claspin protein
  • Protein lengthProtein fragment

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9HAW4
    • SpeciesHuman
    • Molecular weight24 kDa including tags
    • Amino acids1126 to 1330

Specifications

Our Abpromise guarantee covers the use of ab125977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • ReconstitutionReconstitute with water to desired concentration

General Info

  • Alternative names
    • Claspin
    • Claspin homolog
    • claspin homolog (Xenopus laevis)
    • CLSPN
    • CLSPN protein
    • CLSPN_HUMAN
    • hClaspin
    • Hu Claspin
    see all
  • FunctionRequired for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
  • Sequence similaritiesBelongs to the claspin family.
  • DomainThe C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
  • Post-translational
    modifications
    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
  • Cellular localizationNucleus.
  • Information by UniProt

References for Recombinant Human Claspin protein (ab125977)

ab125977 has not yet been referenced specifically in any publications.

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