The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab124320 was purifed using Ni-NTA chromatography. The product is filtered (0.4 µm) but not sterile, hence needs to be filtered by an appropriate sterile filter before using in cell culture.
Additional notesStore lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Avoid storage of lyophilized product at room temperature. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term. Reconstituted protein can be stored at 4°C for a week. Warning: avoid repeated freeze/thaw cycles of solubilized protein.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 4.00 Constituent: 99% Sodium acetate
ReconstitutionAdd 0.1 M Acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/mL. In higher concentrations the solubility of this antigen is limited.
Clostridium perfringens enterotoxin receptor
Clostridium perfringens enterotoxin receptor 1
Williams Beuren syndrome chromosome region 8 protein
Williams-Beuren syndrome chromosomal region 8 protein
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
Involvement in diseaseNote=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the claudin family.