The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab124320 was purifed using Ni-NTA chromatography. The product is filtered (0.4 µm) but not sterile, hence needs to be filtered by an appropriate sterile filter before using in cell culture.
Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Avoid storage of lyophilized product at room temperature. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term. Reconstituted protein can be stored at 4°C for a week. Warning: avoid repeated freeze/thaw cycles of solubilized protein.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 4.00 Constituent: 99% Sodium acetate
Add 0.1 M Acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/mL. In higher concentrations the solubility of this antigen is limited.
Clostridium perfringens enterotoxin receptor
Clostridium perfringens enterotoxin receptor 1
Williams Beuren syndrome chromosome region 8 protein
Williams-Beuren syndrome chromosomal region 8 protein
Plays a major role in tight junction-specific obliteration of the intercellular space.
Involvement in disease
Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.