The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesBest use within three months from the date of receipt of this protein.
Protein concentration is above or equal to 0.05 mg/ml.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.3% Glutathione, 0.79% Tris HCl
Chloride channel 3
Chloride channel protein 3
Chloride channel, voltage-sensitive 3
Chloride transporter ClC 3
Chloride transporter ClC-3
Chloride transporter ClC3
H(+)/Cl(-) exchange transporter 3
FunctionMediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.
Tissue specificityExpressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels. Expressed in bronchus and colon (at protein level).
Sequence similaritiesBelongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily. Contains 2 CBS domains.
DomainIsoform 2 contains a C-terminal PDZ-binding motif mediating the interaction with GOPC.
Cellular localizationMembrane. Early endosome membrane. Late endosome membrane. Cytoplasmic vesicle > secretory vesicle membrane. Isoform 1 is localized mainly in early and late endosomes and Membrane. Early endosome membrane. Late endosome membrane. Golgi apparatus membrane. Isoform 2 partially colocalized with isoform 1 but is mainly enriched in the Golgi.