Recombinant Human CLIC1 protein (ab95486)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR RTETVQKLCP GGQLPFLLYGTEVHTDTNKI EEFLEAVLCP PRYPKLAALN PESNTAGLDI FAKFSAYIKN SNPALNDNLE KGLLKALKVL DNYLTSPLPE EVDETSAEDE GVSQRKFLDG NELTLADCNL LPKLHIVQVV CKKYRGFTIP EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVAKAL K
    • Amino acids1 to 241

Specifications

Our Abpromise guarantee covers the use of ab95486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF-TOF
  • Purity> 90 % SDS-PAGE.
    ab95486 was purified using conventional chromatography techniques
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, pH 8

General Info

  • Alternative names
    • Chloride channel ABP
    • Chloride intracellular channel 1
    • Chloride intracellular channel protein 1
    • CLIC 1
    • CLIC1
    • CLIC1_HUMAN
    • G6
    • hRNCC
    • NCC 27
    • NCC27
    • Nuclear chloride ion channel 27
    • Nuclear chloride ion channel protein
    • p64CLCP
    • Regulatory nuclear chloride ion channel protein
    • RNCC protein
    see all
  • FunctionCan insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
  • Tissue specificityExpression is prominent in heart, placenta, liver, kidney and pancreas.
  • Sequence similaritiesBelongs to the chloride channel CLIC family.
    Contains 1 GST C-terminal domain.
  • DomainMembers of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Post-translational
    modifications
    Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.
  • Cellular localizationNucleus. Nucleus membrane. Cytoplasm. Cell membrane. Mostly in the nucleus including in the nuclear membrane. Small amount in the cytoplasm and the plasma membrane. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain.
  • Information by UniProt

Recombinant Human CLIC1 protein images

  • 15% SDS-PAGE analysis of ab95486 (3ug)

References for Recombinant Human CLIC1 protein (ab95486)

ab95486 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Used this purified protein as a positive control to determine antibody specificity to CLIC1 in western blots. Very low amount (0.3 ug/lane) of protein was sufficient to yield a positive result. Purified CLIC4 protein (ab104744) from Abcam was used as a negative control.
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Submitted Jun 11 2015

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