Recombinant human CNTFR protein (ab191640)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CNTFR protein
See all CNTFR proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA.
Immobilized Human CNTF Receptor alpha at 10 μg/ml (100 μl/well) can bind biotinylated Human CNTF with a linear ranger of 3.2 - 100 ng/ml. -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QRHSPQEAPHVQYERLGSDVTLPCGTANWDAAVTWRVNGTDLAPDLLNGS QLVLHGLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYP KGFYCSWHLPTPTYIPNTFNVTVLHGSKIMVCEKDPALKNRCHIRYMHLF STIKYKVSISVSNALGHNATAITFDEFTIVKPDPPENVVARPVPSNPRRL EVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYA GKEYIIQVAAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAAETTTST TSSLAPPPTTKICDPGELGSGGGP -
Predicted molecular weight
37 kDa including tags -
Amino acids
23 to 346 -
Tags
His tag C-Terminus -
Additional sequence information
AAH01492.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab191640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 5% Trehalose, 95% PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- Ciliary neurotrophic factor receptor
- Ciliary neurotrophic factor receptor alpha
- Ciliary neurotrophic factor receptor subunit alpha
see all -
Function
Binds to CNTF. The alpha subunit provides the receptor specificity. -
Tissue specificity
Nervous system and skeletal muscle. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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Human CNTF R alpha (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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SDS-PAGE analysis of reduced ab191640 stained overnight with Coomassie Blue. DTT-reduced protein migrates as 45-48 kDa due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab191640 has not yet been referenced specifically in any publications.