Recombinant Human Cofilin protein (ab95396)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids2 to 166

Specifications

Our Abpromise guarantee covers the use of ab95396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Western blot

  • Purity> 90 % SDS-PAGE.
    ab95396 is >90% pure as assessed by SDS-PAGE.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

General Info

  • Alternative names
    • 18 kDa phosphoprotein
    • CFL
    • CFL 1
    • CFL1
    • COF1_HUMAN
    • Cofilin
    • Cofilin 1
    • Cofilin 1 non muscle
    • Cofilin non muscle isoform
    • Cofilin-1
    • epididymis secretory protein Li 15
    • HEL-S-15
    • non-muscle isoform
    • p18
    see all
  • FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificityWidely distributed in various tissues.
  • Sequence similaritiesBelongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-3 in resting cells.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt

Recombinant Human Cofilin protein images

  • ab95396 being analysed by 10% SDS-PAGE. Detection utilised Coomassie blue staining.
    Lane 1; 5 µg ab95396.
    Lane 2; Protein markers.

References for Recombinant Human Cofilin protein (ab95396)

ab95396 has not yet been referenced specifically in any publications.

Product Wall

Yes, there is a thrombin cleavage site.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"