The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityInduces Hes-1 in 3T3L-1 cells.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. ab109345 is an 0.2 µm filtered solution.
Induces Hes-1 in 3T3L-1 cells.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Contactin 6 precursor
Neural adhesion molecule
Neural recognition molecule NB 3
Neural recognition molecule NB-3
FunctionContactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination.
Tissue specificityExpressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord.
Sequence similaritiesBelongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Functional Studies - Contactin 6 protein (ab109345)
Induction of Hes-1 with the treatment of hContactin-6-Fc.
A Mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 µg/ml of ab109345 as in indicated time points and each cell lysate was prepared and subjected to Western blot by using anti-Mouse Hes-1 or GAPDH (loading control).
Lane M: Marker
Lane 1: ab109345, 0 minutes
Lane 2: ab109345, 10 minutes
Lane 3: ab109345, 30 minutes
Lane 4: ab109345, 1 h
Lane 5: ab109345, 2 h
Lane 6: ab109345, 4 h
Lane 7: ab109345, 8 h
Lane 8: ab109345, 24 h
References for Recombinant Human Contactin 6 protein (ab109345)
has not yet been referenced specifically in any publications.
Publishing research using ab109345? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"