Recombinant Human Contactin 6 protein (ab109345)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      150 kDa including tags
    • Amino acids
      1 to 999

Specifications

Our Abpromise guarantee covers the use of ab109345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Induces Hes-1 in 3T3L-1 cells.
  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 90 % SDS-PAGE.
    ab109345 is an 0.2 µm filtered solution.
  • Form
    Liquid
  • Additional notes
    Induces Hes-1 in 3T3L-1 cells.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

General Info

  • Alternative names
    • CNTN 6
    • CNTN6
    • CNTN6_HUMAN
    • Contactin 6 precursor
    • Contactin-6
    • Contactin6
    • hNB 3
    • hNB-3
    • hNB3
    • MGC133256
    • NB 3
    • NB3
    • Neural adhesion molecule
    • Neural recognition molecule NB 3
    • Neural recognition molecule NB-3
    see all
  • Function
    Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination.
  • Tissue specificity
    Expressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. Contactin family.
    Contains 4 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

Images

  • Induction of Hes-1 with the treatment of hContactin-6-Fc.
    A Mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 µg/ml of ab109345 as in indicated time points and each cell lysate was prepared and subjected to Western blot by using anti-Mouse Hes-1 or GAPDH (loading control).

    Lane M: Marker
    Lane 1: ab109345, 0 minutes
    Lane 2: ab109345, 10 minutes
    Lane 3: ab109345, 30 minutes
    Lane 4: ab109345, 1 h
    Lane 5: ab109345, 2 h
    Lane 6: ab109345, 4 h
    Lane 7: ab109345, 8 h
    Lane 8: ab109345, 24 h

References

ab109345 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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