The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0
53 kDa putative calcium binding protein
53 kDa putative calcium-binding protein
53 kDa squamous epithelial induced stress protein
53 kDa squamous epithelial-induced stress protein
58 kDa heat shock protein
Chromosome 1 open reading frame 10
Squamous epithelial heat shock protein 53
Tumor related protein
FunctionSurvival factor that participates in the clonogenicity of squamous esophageal epithelium cell lines, attenuates deoxycholic acid (DCA)-induced apoptotic cell death and release of calcium. When overexpressed in oral squamous carcinom cell lines, regulates negatively cell proliferation by the induction of G1 arrest.
Tissue specificitySquamous epithelia cell-specific. Expressed in the esophagus (periphery of the cells of the granular and the upper spinous layers), foreskin (granular and lower cornified cells), scalp skin (granular layer), inner root sheath of the hair follicle and in primary keratinocytes (at protein level). Expressed in the squamous epithelium of the cervix, esophagus, foreskin and larynx. Expressed in the fetal bladder and scalp skin. Expressed at very low levels in the lung, kidney, uterus, skeletal muscle, heart and fetal brain. Undetectable or barely detectable in esophageal and oral squamous cell carcinoma compared with the matched adjacent normal esophageal mucosa. Undetectable or barely detectable in larynx and esophagus from patients with pH-documented laryngopharyngeal reflux (LPR).
Sequence similaritiesBelongs to the S100-fused protein family. Contains 1 EF-hand domain.
DomainThe EF-hand is necessary for the colony survival activity to protect cells from death induced by exposure to DCA.
Cellular localizationCytoplasm. Does not colocalize with TGM1.