Recombinant human COX2 / Cyclooxygenase 2 protein (ab58868)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf21 cells
  • Amino Acid Sequence
    • AccessionP35354
    • SpeciesHuman
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab58868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity: minimum 8,000 units per mg protein
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

    Other

  • FormLiquid
  • Additional notesThis protein is relatively unstable at room temperature; Keep the enzyme on ice (0 °C to 4 °C) at all times.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: 300µM diethyldithio-carbamate (DDC)
    Constituents: 0.1% Tween 20, 80mM Tris HCl, pH 8

    Note: If this preservative (DDC) is undesirable, it can be removed by standard desalting procedures, but the enzyme is unstable in the absence of the preservative and so should be used within one hour.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • COX 2
    • COX-2
    • COX2
    • Cyclooxygenase
    • Cyclooxygenase 2
    • Cyclooxygenase 2b
    • Cyclooxygenase-2
    • Cyclooxygenase2
    • EC 1.14.99.1
    • fj02a10
    • Glucocorticoid-regulated inflammatory cyclooxygenase
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase
    • GRIPGHS
    • hCox 2
    • Macrophage activation-associated marker protein P71/73
    • OTTHUMP00000033524
    • PES-2
    • PGG/HS
    • PGH synthase 2
    • PGH2_HUMAN
    • PGHS 2
    • PGHS-2
    • PGHS2
    • PHS 2
    • PHS II
    • PHS2
    • Prostaglandin endoperoxide synthase 2
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
    • Prostaglandin G/H synthase
    • Prostaglandin G/H synthase 2
    • Prostaglandin G/H synthase 2 precursor
    • Prostaglandin G/H synthase and cyclooxygenase
    • Prostaglandin H2 synthase 2
    • Prostaglandin-endoperoxide synthase 2
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
    • PTGS2
    • ptgs2a
    • TIS10
    • TIS10 protein
    • unp1239
    • wu:fj02a10
    see all
  • FunctionMediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • PathwayLipid metabolism; prostaglandin biosynthesis.
  • Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

Recombinant human COX2 / Cyclooxygenase 2 protein images

  • Standard curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab90345 at 1µg/ml and Detector Antibody ab15191 at 0.5µg/ml.

References for Recombinant human COX2 / Cyclooxygenase 2 protein (ab58868)

ab58868 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"