Recombinant human COX2 / Cyclooxygenase 2 protein (ab58868)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf21 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab58868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: minimum 8,000 units per mg protein
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

    Other

  • Form
    Liquid
  • Additional notes
    This protein is relatively unstable at room temperature; Keep the enzyme on ice (0 °C to 4 °C) at all times.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 1.261% Tris HCl, 0.1% Tween, 0.00675% Diethyldithiocarbamate, 10% Glycerol

    If this preservative (DDC) is undesirable, it can be removed by standard desalting procedures, but the enzyme is unstable in the absence of the preservative and so should be used within one hour.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • COX 2
    • COX-2
    • COX2
    • Cyclooxygenase
    • Cyclooxygenase 2
    • Cyclooxygenase 2b
    • Cyclooxygenase-2
    • Cyclooxygenase2
    • EC 1.14.99.1
    • fj02a10
    • Glucocorticoid-regulated inflammatory cyclooxygenase
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase
    • GRIPGHS
    • hCox 2
    • Macrophage activation-associated marker protein P71/73
    • OTTHUMP00000033524
    • PES-2
    • PGG/HS
    • PGH synthase 2
    • PGH2_HUMAN
    • PGHS 2
    • PGHS-2
    • PGHS2
    • PHS 2
    • PHS II
    • PHS2
    • Prostaglandin endoperoxide synthase 2
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
    • Prostaglandin G/H synthase
    • Prostaglandin G/H synthase 2
    • Prostaglandin G/H synthase 2 precursor
    • Prostaglandin G/H synthase and cyclooxygenase
    • Prostaglandin H2 synthase 2
    • Prostaglandin-endoperoxide synthase 2
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
    • PTGS2
    • ptgs2a
    • TIS10
    • TIS10 protein
    • unp1239
    • wu:fj02a10
    see all
  • Function
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • Pathway
    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Post-translational
    modifications
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

Images

  • Standard curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab90345 at 1µg/ml and Detector Antibody ab15191 at 0.5µg/ml.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References

ab58868 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
This recombinant protein ran one clean band detected by ab15191 Cox2 antibody. Worked perfectly for our needs
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Submitted May 05 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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