Recombinant human Coxsackie Adenovirus Receptor protein (ab168893)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      LSITTPEEMIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPADNQKVDQ VIILYSGDKI YDDYYPDLKGRVHFTSNDLKSGDASINVTNLQLSDIGT YQCKVKKAPGVANKKIHLVVLV KPSGARCYVDGSEEIGSDFKIKCEPK EGSLPLQYEWQKLSDSQKMPTSWLAEMTSSVISV KNASSEYSGTYSCT VRNRVGSDQCLLRLNVVPPSNKAG
    • Molecular weight
      25 kDa including tags
    • Amino acids
      20 to 237
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab168893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Measured by the ability of immobilized ab168893 to support the adhesion of mouse neutrophils. When 50000 cells/well are added to Coxsackie Adenovirus Receptor coated plates (4 µg/mL and 100 µL/well ), approximately 20% - 50% will adhere specifically after 60 minutes at 37?.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C.

    pH: 7.40
    Constituent: 95% PBS
    Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage

General Info

  • Alternative names
    • 46 kD coxsackievirus and adenovirus receptor CAR protein
    • 46 kD coxsackievirus and adenovirus receptor protein
    • CAR
    • CAR4/6
    • Coxsackie virus and adenovirus receptor
    • Coxsackie virus B receptor
    • Coxsackievirus and adenovirus receptor
    • Coxsackievirus B adenovirus receptor
    • Coxsackievirus B-adenovirus receptor
    • CVB3 binding protein
    • CVB3-binding protein
    • Cxadr
    • CXADR Ig like cell adhesion molecule
    • CXAR_HUMAN
    • hCAR
    • HCVADR
    see all
  • Function
    Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.
  • Tissue specificity
    Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain
    The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
    The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
  • Post-translational
    modifications
    N-glycosylated.
    Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
  • Cellular localization
    Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
  • Information by UniProt

Images

  • SDS-PAGE of ab168893 with overnight Coomassie Blue staining.

References

ab168893 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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