The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesStore lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
ReconstitutionAdd deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Corticotropin releasing factor binding protein
Corticotropin releasing hormone binding protein
CRF binding protein
RelevanceCRHBP is a potent stimulator of synthesis and secretion of preopiomelanocortin derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH binding protein which inactivates CRH and which may prevent inappropriate pituitary adrenal stimulation in pregnancy.
Cellular localizationExtracellular space
Recombinant Human CRHBP protein images
SDS-PAGE - CRHBP protein (ab101052)
14% SDS-PAGE analysis of ab101052
Lane 1: Molecular weight markers 97, 66, 45, 31, 21, 14 kDa
Lane 2: ab101052 (reduced and boiled, 5µg/lane)
lane 3: ab101052 (non-reduced and non-boiled, 5µg/lane)
References for Recombinant Human CRHBP protein (ab101052)
has not yet been referenced specifically in any publications.
Publishing research using ab101052? Please let us know so that we can cite the reference in this datasheet.
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