The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityab84064 has been shown to stimulate MAPK phosphorylation in HUVEC cells. 200ng/ml is sufficient to stimulate phosphorylation.
% by SDS-PAGE.
Additional notesab84064 has been shown to stimulate MAPK phosphorylation in HUVEC cells. 200ng/ml is sufficient to stimulate phosphorylation.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, with longer-term sto rage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Cripto 1 growth factor
Cripto-1 growth factor
Cripto1 growth factor
Epidermal growth factor like cripto protein CR1
Epidermal growth factor-like cripto protein CR1
Teratocarcinoma derived growth factor 1
Teratocarcinoma-derived growth factor 1
FunctionCould play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Tissue specificityPreferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Lane 1- MW markers; Lane 2- ab84064 ; Lane 3- ab84064 treated with PNGase F to remove potential N-linked glycans; Lane 4- ab84064 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. A drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
O-fucosylation at Thr-88 has been confirmed.
References for Recombinant human Cripto1 protein (ab84064)
has not yet been referenced specifically in any publications.
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