The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityab84064 has been shown to stimulate MAPK phosphorylation in HUVEC cells. 200ng/ml is sufficient to stimulate phosphorylation.
Additional notesab84064 has been shown to stimulate MAPK phosphorylation in HUVEC cells. 200ng/ml is sufficient to stimulate phosphorylation.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, with longer-term sto rage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Cripto 1 growth factor
Cripto-1 growth factor
Cripto1 growth factor
Epidermal growth factor like cripto protein CR1
Epidermal growth factor-like cripto protein CR1
Teratocarcinoma derived growth factor 1
Teratocarcinoma-derived growth factor 1
FunctionCould play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Tissue specificityPreferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Lane 1- MW markers; Lane 2- ab84064 ; Lane 3- ab84064 treated with PNGase F to remove potential N-linked glycans; Lane 4- ab84064 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. A drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
O-fucosylation at Thr-88 has been confirmed.
References for Recombinant human Cripto1 protein (ab84064)
has not yet been referenced specifically in any publications.
Publishing research using ab84064? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"