The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab101046 was purified by using conventional chromatography.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8.0
Adapter molecule crk
Avian sarcoma virus CT10 (v crk) oncogene homolog
CRK isoform 2
CRK isoform II
Proto oncogene C crk
v crk avian sarcoma virus CT10 oncogene homolog
v crk sarcoma virus CT10 oncogene homolog
v crk sarcoma virus CT10 oncogene homolog (avian)
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
Belongs to the CRK family. Contains 1 SH2 domain. Contains 2 SH3 domains.
The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation. The SH2 domain mediates interaction with SHB.
Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form. Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.