Recombinant human CX3CL1 protein (ab108577)



  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionP78423
    • SpeciesHuman
    • Molecular weight80 kDa including tags
    • Amino acids1 to 339
    • TagsDDDDK tag C-Terminus


Our Abpromise guarantee covers the use of ab108577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityChemotaxis effect of soluble recombinant Human CX3CL1 in THP-1 cells.
  • Applications

    Functional Studies


  • Endotoxin level< 0.100 Eu/µg
  • Purity> 90 % SDS-PAGE.
    ab108577 is 0.2µm filtered
  • FormLiquid
  • Additional notesWorking aliquots are stable for up to 3 months when stored at -20°C.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • A 152E5.2
    • AB030188
    • ABCD 3
    • ABCD3
    • AI848747
    • C-X3-C motif chemokine 1
    • C3Xkine
    • Chemokine (C-X3-C motif) ligand 1
    • Chemokine C X3 C motif ligand 1
    • Chemokine CX3C Motif Ligand 1
    • CX3C membrane anchored chemokine
    • CX3C membrane-anchored chemokine
    • Cx3cl1
    • CXC 3
    • CXC3
    • CXC3C
    • D8Bwg0439e
    • FKN
    • Fractalkine
    • Neurotactin
    • NTN
    • NTT
    • Processed fractalkine
    • SCYD 1
    • SCYD1
    • Small inducible cytokine D1
    • Small inducible cytokine subfamily D (Cys X3 Cys) member 1
    • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
    • Small inducible cytokine subfamily D member 1
    • Small-inducible cytokine D1
    • X3CL1_HUMAN
    see all
  • FunctionThe soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
  • Tissue specificitySmall intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the intercrine delta family.
  • Post-translational
    A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt

Recombinant human CX3CL1 protein images

  • Chemotaxis effect of human CX3CL1-DDDDK (ab108577) in THP-1 Cells.
    Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24 well transwell polystyrene membrane with 8µm size pores. 100ng, 500ng, 1,000ng, 2,500ng and 5,000ng of recombinant CX3CL1 were diluted in RPMI 1640 supplemented with 10mg/ml BSA, respectively. CX3CL1 samples were added to the lower chamber. THP-1 cells (0.5 X 106 cells per well), in the same media with ab108577, were added to the upper chamber. After 2 hour incubation at 37°C in 5% CO2 humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from duplicate wells were enumerated on a hemacytometer by means light microscopy.

References for Recombinant human CX3CL1 protein (ab108577)

ab108577 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab108577.
Please use the links above to contact us or submit feedback about this product.