Recombinant Human CYB5R3 protein (ab119468)

Overview

  • Product nameRecombinant Human CYB5R3 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP00387
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MGSHMFQRST PAITLESPDI KYPLRLIDRE IISHDTRRFR FALPSPQHIL GLPVGQHIYL SARIDGNLVV RPYTPISSDD DKGFVDLVIK VYFKDTHPKF PAGGKMSQYL ESMQIGDTIE FRGPSGLLVY QGKGKFAIRP DKKSNPIIRT VKSVGMIAGG TGITPMLQVI RAIMKDPDDH TVCHLLFANQ TEKDILLRPE LEELRNKHSA RFKLWYTLDR APEAWDYGQG FVNEEMIRDH LPPPEEEPLV LMCGPPPMIQ YACLPNLDHV GHPTERCFVF
    • Molecular weight34 kDa including tags
    • Amino acids27 to 301
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab119468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    ab119468 was purified using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.02% DTT, 10% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names
    • B5R
    • Cyb5r3
    • Cytochrome b5 reductase
    • Cytochrome b5 reductase 3
    • DIA1
    • Diaphorase 1
    • Diaphorase-1
    • NADH cytochrome b5 reductase 3
    • NADH-cytochrome b5 reductase 3 membrane-bound form
    • NADH-cytochrome b5 reductase 3 soluble form
    • NB5R3_HUMAN
    • OTTHUMP00000028761
    • OTTHUMP00000198435
    • OTTHUMP00000198574
    • OTTHUMP00000198662
    • OTTHUMP00000198665
    see all
  • FunctionDesaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
  • Tissue specificityIsoform 2 is expressed at late stages of erythroid maturation.
  • Involvement in diseaseDefects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3) [MIM:250800]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well-tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms.
  • Sequence similaritiesBelongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 FAD-binding FR-type domain.
  • Cellular localizationEndoplasmic reticulum membrane. Mitochondrion outer membrane and Cytoplasm. Produces the soluble form found in erythrocytes.
  • Information by UniProt

Recombinant Human CYB5R3 protein images

  • 15% SDS-PAGE analysis of ab119468 (3µg)

References for Recombinant Human CYB5R3 protein (ab119468)

ab119468 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119468.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"