Recombinant Human Cyclophilin A protein (ab96022)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP62937
    • SpeciesHuman
    • Molecular weight18 kDa including tags
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab96022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab96022 is >99% pure as estimated by SDS-PAGE.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Sodium chloride, 20mM Tris, 0.5mM DTT, pH 8.0

General Info

  • Alternative names
    • Cyclophilin
    • Cyclophilin A
    • CyclophilinA
    • Cyclosporin A binding protein
    • Cyclosporin A-binding protein
    • CYPA
    • CYPH
    • Epididymis secretory sperm binding protein Li 69p
    • HEL S 69p
    • MGC117158
    • MGC12404
    • MGC23397
    • Peptidyl prolyl cis trans isomerase A
    • Peptidyl-prolyl cis-trans isomerase A
    • Peptidylprolyl isomerase A
    • Peptidylprolyl isomerase A (cyclophilin A)
    • PPIA
    • PPIA protein
    • PPIA_HUMAN
    • PPIase A
    • Rotamase A
    • RotamaseA
    • T cell cyclophilin
    see all
  • FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similaritiesBelongs to the cyclophilin-type PPIase family. PPIase A subfamily.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant Human Cyclophilin A protein images

  • Anti-Cyclophilin A antibody (ab42408) at 1/1000 dilution + Recombinant Human Cyclophilin A protein (ab96022) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • 15% polyacrylamide SDS-PAGE gel analysis of ab96022.
    Lane 1: 1 µg ab96022
    Lane 2: 2.5 µg ab96022
    Lane 3: 5 µg ab96022
    Stained with Coomassie Blue (left) or immunoblotted with an anti Cyclophilin A antibody (right).

References for Recombinant Human Cyclophilin A protein (ab96022)

ab96022 has not yet been referenced specifically in any publications.

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