Recombinant human Cyclophilin F protein (ab79186)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHCSKGSGDPSSSSSSGNPLVYLDVDANGKPL GRVVLELKADVVPKTAENFRALCTGEKGFGYKGSTFHRVIPSFMCQAGDF TNHNGTGGKSIYGSRFPDENFTLKHVGPGVLSMANAGPNTNGSQFFICTI KTDWLDGKHVVFGHVKEGMDVVKKIESFGSKSGRTSKKIVITDCGQLS

Associated products

Specifications

Our Abpromise guarantee covers the use of ab79186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific activity is > 250 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant Cyclophilin F (PPIF) protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1deg;C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 1.000 Eu/µg
  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    ab79186 is purified using conventional chromatography techniques. Endotoxin Level: < 1.0 EU per 1µg of protein (determined by LAL method)
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris, 1mM DTT, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Cyclophilin 3
    • cyclophilin D
    • Cyclophilin F
    • Cyp D
    • CyP M
    • CYP3
    • CypD
    • hCyP3
    • mitochondrial
    • Mitochondrial cyclophilin
    • Peptidyl prolyl cis trans isomerase, mitochondral
    • Peptidyl-prolyl cis-trans isomerase F
    • Peptidyl-prolyl cis-trans isomerase F, mitochondrial
    • Peptidylprolyl isomerase F
    • Peptidylprolyl isomerase F (cyclophilin F)
    • PPIase
    • PPIase F
    • Ppif
    • PPIF_HUMAN
    • Rotamase
    • Rotamase F
    see all
  • FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similaritiesBelongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt

Recombinant human Cyclophilin F protein images

  • 15% SDS-PAGE showing ab79186 at approximately 21kDa (3µg).

References for Recombinant human Cyclophilin F protein (ab79186)

This product has been referenced in:
  • Alavian KN  et al. An uncoupling channel within the c-subunit ring of the F1FO ATP synthase is the mitochondrial permeability transition pore. Proc Natl Acad Sci U S A 111:10580-5 (2014). Read more (PubMed: 24979777) »

See 1 Publication for this product

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Thank you for your inquiry. Besides the His-tag, the lab added a thrombin cleavage site (LVPRGS). I hope this information helps. Please contact us with any other questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"