Recombinant Human Cytokeratin 18 protein (ab73638)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Molecular weight48 kDa

Specifications

Our Abpromise guarantee covers the use of ab73638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity> 95 % SDS-PAGE.
    ab73638 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Additional notesReconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCI, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 9.5% Urea, 30mM Tris HCl, 2mM EDTA, 10mM methylammonium chloride, pH 8

  • ReconstitutionReconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • Cell proliferation inducing gene 46 protein
    • Cell proliferation inducing protein 46
    • Cell proliferation-inducing gene 46 protein
    • CK 18
    • CK-18
    • CK18
    • CYK 18
    • CYK18
    • Cytokeratin 18
    • Cytokeratin endo B
    • Cytokeratin-18
    • K 18
    • K18
    • K1C18_HUMAN
    • KA18
    • Keratin 18
    • Keratin 18, type I
    • Keratin D
    • keratin, type I cytoskeletal 18
    • Keratin-18
    • Krt18
    see all
  • FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt

Recombinant Human Cytokeratin 18 protein images

  • Anti-Cytokeratin 18 antibody (ab52948) at 1/250 dilution + Recombinant Human Cytokeratin 18 protein (ab73638) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Recombinant Human Cytokeratin 18 protein (ab73638)

ab73638 has not yet been referenced specifically in any publications.

Product Wall

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Average
Abreviews
Application Sandwich ELISA
We tested this antigen with ab53118 as capture and ab7797 as detection abs.
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Verified customer

Submitted May 22 2015

Abreviews
Application ELISA
We tested this antigen with ab52948 as capture and ab7797 as detection abs. Concentrations above 0.5ug/ml were detectable in this format.
Username

Abcam user community

Verified customer

Submitted Apr 01 2015

Thank you for your enquiry. Some of our products are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise ...

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