Recombinant Human Cytokeratin 8 protein (ab73641)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab73641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab73641 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Additional notesReconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 9.5M Urea, 30mM Tris HCl, 10mM methylammonium chloride, 2mM EDTA, 2mM DTT, pH 8

  • ReconstitutionReconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • CARD2
    • CK 8
    • CK-8
    • CK8
    • CYK8
    • CYKER
    • Cytokeratin endo A
    • Cytokeratin-8
    • DreK8
    • EndoA
    • K2C8
    • K2C8_HUMAN
    • K8
    • Keratin 8
    • Keratin type II cytoskeletal 8
    • Keratin, type II cytoskeletal 8
    • Keratin-8
    • KO
    • Krt 2.8
    • KRT8
    • MGC118110
    • MGC174782
    • MGC53564
    • MGC85764
    • sb:cb186
    • Type-II keratin Kb8
    see all
  • FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in diseaseCirrhosis
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt

References for Recombinant Human Cytokeratin 8 protein (ab73641)

ab73641 has not yet been referenced specifically in any publications.

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