Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      58 kDa including tags
    • Amino acids
      1 to 296
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab112279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    useful for Antibody Production and Protein Array
  • Applications

    Western blot

    SDS-PAGE

    ELISA

    Peptide Array

  • Form
    Liquid
  • Additional notes
    Best use within three months from the date of receipt of this protein.useful for Antibody Production and Protein Array
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione
    Note: Reduced glutathione

General Info

  • Alternative names
    • DAZ homolog
    • DAZ like autosomal
    • DAZ-like autosomal
    • DAZH
    • DAZL
    • DAZL 1
    • DAZL_HUMAN
    • DAZL1
    • DAZLA
    • Deleted in azoospermia like
    • Deleted in azoospermia like 1
    • Deleted in azoospermia like autosomal
    • Deleted in azoospermia-like
    • Deleted in azoospermia-like 1
    • Germline specific RNA binding protein
    • MGC26406
    • Spermatogenesis gene on the Y like autosomal
    • SPGY like autosomal
    • SPGY-like-autosomal
    • SPGYLA
    • Tpx2
    see all
  • Function
    RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
  • Tissue specificity
    Testis specific.
  • Sequence similarities
    Belongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
  • Information by UniProt

Recombinant Human DAZL protein images

  • 12.5% SDS-PAGE Stained with Coomassie Blue with recombinant protein.
  • Anti-DAZL antibody (ab34139) at 1 µg/ml + Recombinant Human DAZL protein (ab112279) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58.19 kDa


    Exposure time : 10 seconds

References for Recombinant Human DAZL protein (ab112279)

ab112279 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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