Recombinant Human DDAH1 protein (ab99863)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGSMAGLGHPAAFGRATHAVVRALPESLGQ HALRSAKGEEVDVARAERQHQLYVGVLGSKLGLQVVELPADESLPDCVFV EDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLD GGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSF CSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNI PNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSV LINKKVDS
    • Molecular weight
      34 kDa including tags
    • Amino acids
      1 to 285
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    ab99863 is purified using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • DDAH
    • DDAH I
    • DDAH-1
    • DDAH1
    • DDAH1_HUMAN
    • DDAHI
    • Dimethylargininase 1
    • Dimethylargininase-1
    • Dimethylarginine dimethylaminohydrolase 1
    • N(G)
    • N(G)-dimethylarginine dimethylaminohydrolase 1
    • NG NG dimethylarginine dimethylaminohydrolase
    see all
  • Function
    Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
  • Tissue specificity
    Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
  • Sequence similarities
    Belongs to the DDAH family.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab99863 (3µg).

References

ab99863 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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We have:

DDAH1 protein (http://www.abcam.com/ab99863), Recombinant full length Human DDAH1 (amino acids 1-285) with an N terminal His tag.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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