Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      154 kDa including tags
    • Amino acids
      1 to 1140
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab114333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • Damage specific DNA binding protein 1
    • Damage-specific DNA-binding protein 1
    • DDB 1
    • DDB p127 subunit
    • Ddb1
    • DDB1_HUMAN
    • DDBa
    • DNA damage binding protein 1
    • DNA damage-binding protein 1
    • DNA damage-binding protein a
    • HBV X-associated protein 1
    • UV damaged DNA binding factor
    • UV damaged DNA binding protein 1
    • UV DDB 1
    • UV DDB1
    • UV-damaged DNA-binding factor
    • UV-damaged DNA-binding protein 1
    • UV-DDB 1
    • X associated protein 1
    • XAP 1
    • XAP-1
    • XAP1
    • Xeroderma pigmentosum group E complementing protein
    • Xeroderma pigmentosum group E-complementing protein
    • XPCe
    • XPE
    • XPE BF
    • XPE binding factor
    • XPE-BF
    • XPE-binding factor
    see all
  • Function
    Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the DDB1 family.
  • Post-translational
    modifications
    Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis.
  • Cellular localization
    Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage.
  • Information by UniProt

Images

  • ab114333 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

References

ab114333 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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