The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Coating ab169898 at 5-10 µg / well (6 well plate) in a specific culture medium may be used for studying Human cells activation / differentiation in vitro.
>90% by SDS-PAGE. ab169898 was expressed in E.coli as inclusion bodies, then refolded using “temperature shift inclusion body refolding” technology, chromatographically purified and sterile-filtered.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 8.00 Constituent: 0.32% Tris HCl Note: Contains NaCl, KCl, EDTA, arginine, DTT and glycerol.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Bone proteoglycan II
Dermatan sulphate proteoglycans II
Proteoglycan core protein
Small leucine rich protein 1B
May affect the rate of fibrils formation.
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Secreted > extracellular space > extracellular matrix.