Overview

  • Product name
    Recombinant human DEP1 protein
  • Protein length
    Protein fragment

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      59 kDa
    • Amino acids
      1024 to 1306
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab42588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: 268 nmole/min/µg. Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and DEP1. Assay was done at 30&Deg;C for 10 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1

  • Applications

    Phosphatase Activity

    Inhibition Assay

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 75mM Sodium chloride, 25mM Tris HCl, 2mM EDTA, 10mM Glutathione, 1mM DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CD 148
    • CD148
    • CD148 antigen
    • Density enhanced phosphatase 1
    • Density-enhanced phosphatase 1
    • DEP 1
    • DEP-1
    • HPTP eta
    • HPTPeta
    • Human density enhanced phosphatase 1
    • Protein tyrosine phosphatase eta
    • Protein tyrosine phosphatase receptor type J
    • Protein tyrosine phosphatase receptor type J polypeptide
    • Protein-tyrosine phosphatase eta
    • Protein-tyrosine phosphatase receptor type J
    • PTPJ
    • Ptprj
    • PTPRJ_HUMAN
    • R PTP Eta
    • R-PTP-eta
    • R-PTP-J
    • Receptor type tyrosine protein phosphatase eta
    • Receptor-type tyrosine-protein phosphatase eta
    • SCC 1
    • SCC1
    • susceptibility to colon cancer 1, mouse, homolog of
    see all
  • Function
    Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
  • Tissue specificity
    Expressed in the promyelocytic cell line HL60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the interleukin-3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
    Contains 9 fibronectin type-III domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane. Cell projection > ruffle membrane. Cell junction. After T cell stimulation, it is temporarily excluded from immunological synapses.
  • Information by UniProt

References

ab42588 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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