Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceTLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH

Specifications

Our Abpromise guarantee covers the use of ab82115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200ng/ml.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Purity is greater than 97% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Dickkopf 1
    • Dickkopf 1 homolog
    • Dickkopf 1 like
    • Dickkopf homolog 1
    • Dickkopf like protein 1
    • Dickkopf related protein 1
    • Dickkopf WNT signaling pathway inhibitor 1
    • Dickkopf-1
    • Dickkopf-related protein 1
    • DKK 1
    • Dkk-1
    • Dkk1
    • DKK1_HUMAN
    • hDkk 1
    • hDkk-1
    • SK
    see all
  • FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificityPlacenta.
  • Sequence similaritiesBelongs to the dickkopf family.
  • DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human DKK1 protein (ab82115)

ab82115 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"