The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityInhibits adipogenesis of mesenchymal stem cells.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. ab108576 is 0.2µm filtered
Additional notesWorking aliquots are stable for up to 3 months when stored at -20°C.
Inhibits adipogenesis of mesenchymal stem cells.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Delta (Drosophila) like 1
Delta like 1
Delta like 1 (Drosophila)
Delta like 1 protein
Delta like protein 1
Delta like protein 1 precursor
Delta, Drosophila, homolog of 1
Delta-like protein 1
Drosophila Delta homolog 1
FunctionActs as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Tissue specificityExpressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
Adipogenesis inhibition of MSCs.
MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1µM Dexamethasone, 0.5mM IBMX, 10µg/m lnsulin, 100µM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were first incubated with a solution of anti-FLAG® antibody (10µg/ml) in PBS for 30 mins at 37°C. Coated plates were blocked with growth medium for at least 30mins, incubated with solution of ab108576 (5µg/ml) or mCD137L-FLAG® (5µg/ml) in growth medium for 2 hours at 37°C. Plates were then used to differentiate MSCs.
References for Recombinant Human DLL1 protein (ab108576)
has not yet been referenced specifically in any publications.
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